You are watching: Why are plates incubated upside down
Petri key were an initial used through German physician Julius Petri in 1887. He covered one culture plate to another plate to protect against the contamination. Before him, the culture plates were extended with the glass bell jars.
All of we recognize that the Petriplates are incubated in reverse position however this inquiry arises in ours mind that why this plates space incubated in reverse position? ns got many questions day-to-day from medicine professionals and this inquiry is asked an extremely frequently. There are following advantages during incubation once we incubate the key in turning back position.
1. Once the plates space incubated in the regular position, water evaporation native media occurs. These vapors condense on the lid that the Petri dish and drops autumn on the colonies arisen on the media surface. This reasons colony mix-up through each other and spread transparent the key surface. This create the trouble in counting and proper decision of microbial count.
2. The evaporation of water native media can cause media dryness that can influence the microbial growth but when the plates space incubated in an reverse position, the rate of evaporation to reduce that results in suitable microbial growth. Therefore, Petri dishes with media can additionally be stored because that a longer duration in an reverse position.
3. The lid of Petri dishes might contain any contamination that spreads top top the media and grows through the sample microbes. This may create an error in microbial count determination.
4. It is straightforward to handle the turning back Petri dishes due to the fact that the lid the the Petri dish might open during managing when incubated in regular position and it may reason contamination from air. We brand the Petri dishes in ~ the bottom part because lid might exchange with various other Petri dishes creating confusion and also inverted position makes it easy to read the labeling of Petriplates.
Above benefits are noticed by me during my functioning in pharmaceuticals. You can also include yours’ in the comment section.
Ankur Choudhary is India"s first professional medicine blogger, author and also founder of medicine Guidelines, a widely-read medicine blog because 2008. Sign-up for the complimentary email updates because that your day-to-day dose of medicine tips..moc.enilediugamrahp
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